ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons

An Jing Ren; Kai Wang; Huan Zhang; Anjun Liu; Xianhua Ma; Qing Liang; Dongmei Cao; John N. Wood; David Z. He; Yu Qiang Ding; Wen Jun Yuan; Zhifang Xie; Weiping J. Zhang

doi:10.1038/ncomms5984

ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons

An Jing Ren, Kai Wang, Huan Zhang, Anjun Liu, Xianhua Ma, Qing Liang, Dongmei Cao, John N. Wood, David Z. He, Yu Qiang Ding, Wen Jun Yuan, Zhifang Xie, Weiping J. Zhang

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In mammals, pain sensation is initiated by the detection of noxious stimuli through specialized transduction ion channels and receptors in nociceptive sensory neurons. Transient receptor potential (TRP) channels are the key sensory transducers that confer nociceptors distinct sensory modalities. However, the regulatory mechanisms about their expression are poorly defined. Here we show that the zinc-finger protein ZBTB20 regulates TRP channels expression in nociceptors. ZBTB20 is highly expressed in nociceptive sensory neurons of dorsal root ganglia. Disruption of ZBTB20 in nociceptors led to a marked decrease in the expression levels of TRPV1, TRPA1 and TRPM8 and the response of calcium flux and whole-cell currents evoked by their respective specific agonists. Phenotypically, the mice lacking ZBTB20 specifically in nociceptors showed a defect in nociception and pain sensation in response to thermal, mechanical and inflammatory stimulation. Our findings point to ZBTB20 as a critical regulator of nociception and pain sensation by modulating TRP channels expression in nociceptors.

Original language	English (US)
Article number	4984
Journal	Nature Communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5984
State	Published - 2014

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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@article{16f2897482d54e05b46eab28b352ed17,

title = "ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons",

abstract = "In mammals, pain sensation is initiated by the detection of noxious stimuli through specialized transduction ion channels and receptors in nociceptive sensory neurons. Transient receptor potential (TRP) channels are the key sensory transducers that confer nociceptors distinct sensory modalities. However, the regulatory mechanisms about their expression are poorly defined. Here we show that the zinc-finger protein ZBTB20 regulates TRP channels expression in nociceptors. ZBTB20 is highly expressed in nociceptive sensory neurons of dorsal root ganglia. Disruption of ZBTB20 in nociceptors led to a marked decrease in the expression levels of TRPV1, TRPA1 and TRPM8 and the response of calcium flux and whole-cell currents evoked by their respective specific agonists. Phenotypically, the mice lacking ZBTB20 specifically in nociceptors showed a defect in nociception and pain sensation in response to thermal, mechanical and inflammatory stimulation. Our findings point to ZBTB20 as a critical regulator of nociception and pain sensation by modulating TRP channels expression in nociceptors.",

author = "Ren, {An Jing} and Kai Wang and Huan Zhang and Anjun Liu and Xianhua Ma and Qing Liang and Dongmei Cao and Wood, {John N.} and He, {David Z.} and Ding, {Yu Qiang} and Yuan, {Wen Jun} and Zhifang Xie and Zhang, {Weiping J.}",

note = "Funding Information: We are grateful to Dr Qiufu Ma for providing mRNA probe constructs. This work was supported by the National Natural Science Foundation of China grants 31025013 (to W.J.Z.), 31270814 (to Z.X.), 81130084 (to W.J.Z.), 31271221 (to A.-J.R.), 31030034 (to Y.-Q.D.) and 31128008 (to D.Z.H.) and by the National Key Basic Research Program of China grants 2012CB524904 (to W.J.Z.) and 2013CB53603 (to W.J.Z.). A.-J.R. was supported in part by Shanghai Postdoctoral Scientific Program (12R21418000). Publisher Copyright: {\textcopyright} 2014 Macmillan Publishers Limited.",

year = "2014",

doi = "10.1038/ncomms5984",

language = "English (US)",

volume = "5",

journal = "Nature Communications",

issn = "2041-1723",

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TY - JOUR

T1 - ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons

AU - Ren, An Jing

AU - Wang, Kai

AU - Zhang, Huan

AU - Liu, Anjun

AU - Ma, Xianhua

AU - Liang, Qing

AU - Cao, Dongmei

AU - Wood, John N.

AU - He, David Z.

AU - Ding, Yu Qiang

AU - Yuan, Wen Jun

AU - Xie, Zhifang

AU - Zhang, Weiping J.

N1 - Funding Information: We are grateful to Dr Qiufu Ma for providing mRNA probe constructs. This work was supported by the National Natural Science Foundation of China grants 31025013 (to W.J.Z.), 31270814 (to Z.X.), 81130084 (to W.J.Z.), 31271221 (to A.-J.R.), 31030034 (to Y.-Q.D.) and 31128008 (to D.Z.H.) and by the National Key Basic Research Program of China grants 2012CB524904 (to W.J.Z.) and 2013CB53603 (to W.J.Z.). A.-J.R. was supported in part by Shanghai Postdoctoral Scientific Program (12R21418000). Publisher Copyright: © 2014 Macmillan Publishers Limited.

PY - 2014

Y1 - 2014

N2 - In mammals, pain sensation is initiated by the detection of noxious stimuli through specialized transduction ion channels and receptors in nociceptive sensory neurons. Transient receptor potential (TRP) channels are the key sensory transducers that confer nociceptors distinct sensory modalities. However, the regulatory mechanisms about their expression are poorly defined. Here we show that the zinc-finger protein ZBTB20 regulates TRP channels expression in nociceptors. ZBTB20 is highly expressed in nociceptive sensory neurons of dorsal root ganglia. Disruption of ZBTB20 in nociceptors led to a marked decrease in the expression levels of TRPV1, TRPA1 and TRPM8 and the response of calcium flux and whole-cell currents evoked by their respective specific agonists. Phenotypically, the mice lacking ZBTB20 specifically in nociceptors showed a defect in nociception and pain sensation in response to thermal, mechanical and inflammatory stimulation. Our findings point to ZBTB20 as a critical regulator of nociception and pain sensation by modulating TRP channels expression in nociceptors.

AB - In mammals, pain sensation is initiated by the detection of noxious stimuli through specialized transduction ion channels and receptors in nociceptive sensory neurons. Transient receptor potential (TRP) channels are the key sensory transducers that confer nociceptors distinct sensory modalities. However, the regulatory mechanisms about their expression are poorly defined. Here we show that the zinc-finger protein ZBTB20 regulates TRP channels expression in nociceptors. ZBTB20 is highly expressed in nociceptive sensory neurons of dorsal root ganglia. Disruption of ZBTB20 in nociceptors led to a marked decrease in the expression levels of TRPV1, TRPA1 and TRPM8 and the response of calcium flux and whole-cell currents evoked by their respective specific agonists. Phenotypically, the mice lacking ZBTB20 specifically in nociceptors showed a defect in nociception and pain sensation in response to thermal, mechanical and inflammatory stimulation. Our findings point to ZBTB20 as a critical regulator of nociception and pain sensation by modulating TRP channels expression in nociceptors.

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SN - 2041-1723

VL - 5

JO - Nature Communications

JF - Nature Communications

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ER -

ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons

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