Two-Dimensional Graphene as a Matrix for MALDI Imaging Mass Spectrometry

William L. Friesen; Brian J. Schultz; Joel F. Destino; Theodore E.G. Alivio; Joseph R. Steet; Sarbajit Banerjee; Troy D. Wood

doi:10.1007/s13361-015-1243-6

Two-Dimensional Graphene as a Matrix for MALDI Imaging Mass Spectrometry

William L. Friesen, Brian J. Schultz, Joel F. Destino, Theodore E.G. Alivio, Joseph R. Steet, Sarbajit Banerjee, Troy D. Wood

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Here, a matrix using two-dimensional (2D) graphene is demonstrated for the first time in the context of MALDI IMS using a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Although graphene flakes have been used previously in MALDI, it is described here how a single 2D layer of graphene is applied directly on top of rat brain sections and soybean leaves. Several classes of molecules are desorbed and ionized off of the surface of the tissues examined using 2D graphene, with minimal background interference from the matrix. Moreover, no solvents are employed in application of 2D graphene, eliminating the potential for analyte diffusion in liquid droplets during matrix application. Because 2D graphene is an elemental form of carbon, an additional advantage is its high compatibility with the long duration needed for many IMS experiments. (Figure Presented).

Original language	English (US)
Pages (from-to)	1963-1966
Number of pages	4
Journal	Journal of the American Society for Mass Spectrometry
Volume	26
Issue number	11
DOIs	https://doi.org/10.1007/s13361-015-1243-6
State	Published - Nov 1 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Spectroscopy

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@article{1e66f0da99604723996825813cd23711,

title = "Two-Dimensional Graphene as a Matrix for MALDI Imaging Mass Spectrometry",

abstract = "Here, a matrix using two-dimensional (2D) graphene is demonstrated for the first time in the context of MALDI IMS using a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Although graphene flakes have been used previously in MALDI, it is described here how a single 2D layer of graphene is applied directly on top of rat brain sections and soybean leaves. Several classes of molecules are desorbed and ionized off of the surface of the tissues examined using 2D graphene, with minimal background interference from the matrix. Moreover, no solvents are employed in application of 2D graphene, eliminating the potential for analyte diffusion in liquid droplets during matrix application. Because 2D graphene is an elemental form of carbon, an additional advantage is its high compatibility with the long duration needed for many IMS experiments. (Figure Presented).",

author = "Friesen, {William L.} and Schultz, {Brian J.} and Destino, {Joel F.} and Alivio, {Theodore E.G.} and Steet, {Joseph R.} and Sarbajit Banerjee and Wood, {Troy D.}",

note = "Funding Information: The project described was supported by Award Number S10RR029517 from the National Center for Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health. AFM was performed using an instrument acquired through support from the National Science Foundation under MRI: 1126301. The authors thank Dr. Alexis Thompson for donation of the rat brain tissue and use of a microtome, and Roger and Beverly Wood for permitting the harvest of soybean leaves. Publisher Copyright: {\textcopyright} 2015 American Society for Mass Spectrometry.",

year = "2015",

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AU - Friesen, William L.

AU - Schultz, Brian J.

AU - Destino, Joel F.

AU - Alivio, Theodore E.G.

AU - Steet, Joseph R.

AU - Banerjee, Sarbajit

AU - Wood, Troy D.

N1 - Funding Information: The project described was supported by Award Number S10RR029517 from the National Center for Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health. AFM was performed using an instrument acquired through support from the National Science Foundation under MRI: 1126301. The authors thank Dr. Alexis Thompson for donation of the rat brain tissue and use of a microtome, and Roger and Beverly Wood for permitting the harvest of soybean leaves. Publisher Copyright: © 2015 American Society for Mass Spectrometry.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Here, a matrix using two-dimensional (2D) graphene is demonstrated for the first time in the context of MALDI IMS using a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Although graphene flakes have been used previously in MALDI, it is described here how a single 2D layer of graphene is applied directly on top of rat brain sections and soybean leaves. Several classes of molecules are desorbed and ionized off of the surface of the tissues examined using 2D graphene, with minimal background interference from the matrix. Moreover, no solvents are employed in application of 2D graphene, eliminating the potential for analyte diffusion in liquid droplets during matrix application. Because 2D graphene is an elemental form of carbon, an additional advantage is its high compatibility with the long duration needed for many IMS experiments. (Figure Presented).

AB - Here, a matrix using two-dimensional (2D) graphene is demonstrated for the first time in the context of MALDI IMS using a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Although graphene flakes have been used previously in MALDI, it is described here how a single 2D layer of graphene is applied directly on top of rat brain sections and soybean leaves. Several classes of molecules are desorbed and ionized off of the surface of the tissues examined using 2D graphene, with minimal background interference from the matrix. Moreover, no solvents are employed in application of 2D graphene, eliminating the potential for analyte diffusion in liquid droplets during matrix application. Because 2D graphene is an elemental form of carbon, an additional advantage is its high compatibility with the long duration needed for many IMS experiments. (Figure Presented).

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Two-Dimensional Graphene as a Matrix for MALDI Imaging Mass Spectrometry

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