The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

M. C. Lin; Y. Salomon; M. Rendell; M. Rodbell

The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

M. C. Lin, Y. Salomon, M. Rendell, M. Rodbell

Department of Medicine

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47 Scopus citations

Abstract

The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg²⁺], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

Original language	English (US)
Pages (from-to)	4246-4252
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	250
Issue number	11
State	Published - 1975

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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abstract = "The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.",

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AU - Lin, M. C.

AU - Salomon, Y.

AU - Rendell, M.

AU - Rodbell, M.

PY - 1975

Y1 - 1975

N2 - The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

AB - The kinetic characteristics of substrate utilization by hepatic adenylate cyclase were investigated under a variety of incubation conditions, including variations in pH, [substrate], [Mg2+], and in the absence or presence of glucagon. Activities were compared with ATP and 5' adenylylimidodiphosphate [App(NH)p] as substrates. The K(m) for both substrates was about 50 μM; V(max) given with App(NH)p was about 40% lower than obtained with ATP as substrate.

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IS - 11

ER -

The hepatic adenylate cyclase system. II. Substrate binding and utilization and the effects of magnesium ion and pH

Abstract

All Science Journal Classification (ASJC) codes

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