Studying inner ear protein-protein interactions using FRET and FLIM

Richard Hallworth; Benjamin Currall; Michael G. Nichols; Xudong Wu; Jian Zuo

doi:10.1016/j.brainres.2006.02.076

Studying inner ear protein-protein interactions using FRET and FLIM

Richard Hallworth, Benjamin Currall, Michael G. Nichols, Xudong Wu, Jian Zuo

Research output: Contribution to journal › Review article › peer-review

20 Scopus citations

Abstract

Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies.

Original language	English (US)
Pages (from-to)	122-131
Number of pages	10
Journal	Brain Research
Volume	1091
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2006.02.076
State	Published - May 26 2006

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2006.02.076

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title = "Studying inner ear protein-protein interactions using FRET and FLIM",

abstract = "Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies.",

author = "Richard Hallworth and Benjamin Currall and Nichols, {Michael G.} and Xudong Wu and Jian Zuo",

note = "Funding Information: Supported by DC 06471, ALSAC, and CA21765 (to J.Z.), DC 02053 (to R.H.), RR17417-01 (to Creighton University), and NSF-EPSCoR EPS-0346476 (to RH). R.H. is the Director of the Nebraska Center for Cell Biology, where the images in this review were obtained. We thank LeAnn Tiede, Creighton University, for help in implementing FLIM, and Ammasi Periasamy and Richard Day, University of Virginia, for help in understanding FRET methods. We also thank Heather C. Jensen-Smith and Wade Bell for useful comments on the manuscript. ",

year = "2006",

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doi = "10.1016/j.brainres.2006.02.076",

language = "English (US)",

volume = "1091",

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AU - Hallworth, Richard

AU - Currall, Benjamin

AU - Nichols, Michael G.

AU - Wu, Xudong

AU - Zuo, Jian

N1 - Funding Information: Supported by DC 06471, ALSAC, and CA21765 (to J.Z.), DC 02053 (to R.H.), RR17417-01 (to Creighton University), and NSF-EPSCoR EPS-0346476 (to RH). R.H. is the Director of the Nebraska Center for Cell Biology, where the images in this review were obtained. We thank LeAnn Tiede, Creighton University, for help in implementing FLIM, and Ammasi Periasamy and Richard Day, University of Virginia, for help in understanding FRET methods. We also thank Heather C. Jensen-Smith and Wade Bell for useful comments on the manuscript.

PY - 2006/5/26

Y1 - 2006/5/26

N2 - Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies.

AB - Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies.

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Studying inner ear protein-protein interactions using FRET and FLIM

Abstract

All Science Journal Classification (ASJC) codes

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