TY - JOUR
T1 - Role of Prostanoid Production and Receptors in the Regulation of Retinal Endogenous Amino Acid Neurotransmitters by 8-Isoprostaglandin E 2, Ex Vivo
AU - Zhao, Min
AU - Destache, Christopher J.
AU - Ohia, Sunny E.
AU - Opere, Catherine A.
N1 - Funding Information:
Acknowledgments The authors would like to thank J. F. O’Neill Packing Company, Omaha, Nebraska for their generous donation of cow eyeballs. Supported by NIH NEI 013967.
PY - 2009/12
Y1 - 2009/12
N2 - The role of enzymes and receptors of the prostanoid pathway in the inhibitory effect of 8-isoprostaglandin E 2 (8-isoPGE 2) on endogenous amino acid neurotransmitter levels was examined, ex vivo. Freshly isolated bovine eyeballs were injected intravitreally with IsoPs, incubated in Krebs buffer for 30 min and retina prepared for HPLC-ECD detection of amino acids. 8-isoPGE 2 attenuated retinal glutamate and its metabolite, glutamine and glycine in a concentration-dependent manner. The non-selective cyclooxygenase (COX)-inhibitor, flurbiprofen, COX-2 selective inhibitor, NS-398 and thromboxane (Tx) synthase inhibitor, furegrelate had no effect on both basal amino acid levels and the inhibitory effects of 8-isoPGE 2 (1-100 μM) on the retinal amino acids. Whereas the TP-receptor antagonist SQ-29548(10 μM) exhibited no effect, SC-19220(EP 1; 30 μM), AH-6809(EP 1-3; 30 μM) and AH-23848(EP 4; 30 μM) reversed the inhibitory effects of 8-isoPGE 2 (0.01-100 μM) on glutamate, glutamine and glycine levels. We conclude that prostanoid EP-receptors regulate the inhibitory effect of 8-isoPGE 2 on basal levels of endogenous amino acids in bovine retina, ex vivo.
AB - The role of enzymes and receptors of the prostanoid pathway in the inhibitory effect of 8-isoprostaglandin E 2 (8-isoPGE 2) on endogenous amino acid neurotransmitter levels was examined, ex vivo. Freshly isolated bovine eyeballs were injected intravitreally with IsoPs, incubated in Krebs buffer for 30 min and retina prepared for HPLC-ECD detection of amino acids. 8-isoPGE 2 attenuated retinal glutamate and its metabolite, glutamine and glycine in a concentration-dependent manner. The non-selective cyclooxygenase (COX)-inhibitor, flurbiprofen, COX-2 selective inhibitor, NS-398 and thromboxane (Tx) synthase inhibitor, furegrelate had no effect on both basal amino acid levels and the inhibitory effects of 8-isoPGE 2 (1-100 μM) on the retinal amino acids. Whereas the TP-receptor antagonist SQ-29548(10 μM) exhibited no effect, SC-19220(EP 1; 30 μM), AH-6809(EP 1-3; 30 μM) and AH-23848(EP 4; 30 μM) reversed the inhibitory effects of 8-isoPGE 2 (0.01-100 μM) on glutamate, glutamine and glycine levels. We conclude that prostanoid EP-receptors regulate the inhibitory effect of 8-isoPGE 2 on basal levels of endogenous amino acids in bovine retina, ex vivo.
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U2 - 10.1007/s11064-009-0013-x
DO - 10.1007/s11064-009-0013-x
M3 - Article
C2 - 19513831
AN - SCOPUS:70450245456
SN - 0364-3190
VL - 34
SP - 2170
EP - 2180
JO - Neurochemical Research
JF - Neurochemical Research
IS - 12
ER -