Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope

Chad E. Bigelow; Curtis J. Harkrider; David L. Conover; Thomas H. Foster; Irene Georgakoudi; Soumya Mitra; Michael G. Nichols; Milind Rajadhyaksha

doi:10.1063/1.1382637

Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope

Chad E. Bigelow, Curtis J. Harkrider, David L. Conover, Thomas H. Foster, Irene Georgakoudi, Soumya Mitra, Michael G. Nichols, Milind Rajadhyaksha

Department of Physics

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We describe the design and implementation of an inverted laser scanning confocal fluorescence microscope utilizing the commercial Nikon Diaphot TMD platform. An external confocal scanner was retrofitted through the video side port of the Diaphot. With 10×, 0.5 NA dry and 60×, 1.4 NA oil immersion objectives, the depth discrimination is 5.8 μm and 0.8 μm, respectively, as determined by derivatives of fluorescence edge responses measured in liquid samples of rhodamine 6G dissolved in DMSO. We present sample edge response curves and representative confocal fluorescence images of tumor cells in monolayer culture.

Original language	English (US)
Pages (from-to)	3407-3410
Number of pages	4
Journal	Review of Scientific Instruments
Volume	72
Issue number	8
DOIs	https://doi.org/10.1063/1.1382637
State	Published - Aug 1 2001

All Science Journal Classification (ASJC) codes

Instrumentation

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10.1063/1.1382637

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abstract = "We describe the design and implementation of an inverted laser scanning confocal fluorescence microscope utilizing the commercial Nikon Diaphot TMD platform. An external confocal scanner was retrofitted through the video side port of the Diaphot. With 10×, 0.5 NA dry and 60×, 1.4 NA oil immersion objectives, the depth discrimination is 5.8 μm and 0.8 μm, respectively, as determined by derivatives of fluorescence edge responses measured in liquid samples of rhodamine 6G dissolved in DMSO. We present sample edge response curves and representative confocal fluorescence images of tumor cells in monolayer culture.",

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AU - Bigelow, Chad E.

AU - Harkrider, Curtis J.

AU - Conover, David L.

AU - Foster, Thomas H.

AU - Georgakoudi, Irene

AU - Mitra, Soumya

AU - Nichols, Michael G.

AU - Rajadhyaksha, Milind

PY - 2001/8/1

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AB - We describe the design and implementation of an inverted laser scanning confocal fluorescence microscope utilizing the commercial Nikon Diaphot TMD platform. An external confocal scanner was retrofitted through the video side port of the Diaphot. With 10×, 0.5 NA dry and 60×, 1.4 NA oil immersion objectives, the depth discrimination is 5.8 μm and 0.8 μm, respectively, as determined by derivatives of fluorescence edge responses measured in liquid samples of rhodamine 6G dissolved in DMSO. We present sample edge response curves and representative confocal fluorescence images of tumor cells in monolayer culture.

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Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope

Abstract

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