TY - JOUR
T1 - Counter-regulatory paracrine actions of FGF-23 and 1,25(OH)2D in macrophages
AU - Han, Xiaobin
AU - Li, Linqiang
AU - Yang, Jiancheng
AU - King, Gwendalyn
AU - Xiao, Zhousheng
AU - Quarles, Leigh Darryl
N1 - Funding Information:
This work was supported by grant R01-AR045955 to LDQ from the National Institutes of Health. Jiancheng Yang was supported in part by China Scholarship Council (No.201308210088). Linqiang Li was supported in part by Harbin Medical University, China.
Publisher Copyright:
© 2015 Federation of European Biochemical Societies.
PY - 2016/1
Y1 - 2016/1
N2 - Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages. NF-ΚB and JAK/STAT1 pathways mediated the increased transcription of FGF-23 in response to M1 polarization. FGF-23 stimulated TNF-α, but not IL-6, expression in M0 macrophages and suppressed Arginase-1 expression in M2 macrophages through FGFR-mediated mechanisms. 1,25(OH)2D stimulated Arginase-1 expression and inhibited FGF-23 stimulation of TNF-α. FGF-23 has proinflammatory paracrine functions and counter-regulatory actions to 1,25(OH)2D on innate immune responses.
AB - Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages. NF-ΚB and JAK/STAT1 pathways mediated the increased transcription of FGF-23 in response to M1 polarization. FGF-23 stimulated TNF-α, but not IL-6, expression in M0 macrophages and suppressed Arginase-1 expression in M2 macrophages through FGFR-mediated mechanisms. 1,25(OH)2D stimulated Arginase-1 expression and inhibited FGF-23 stimulation of TNF-α. FGF-23 has proinflammatory paracrine functions and counter-regulatory actions to 1,25(OH)2D on innate immune responses.
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U2 - 10.1002/1873-3468.12040
DO - 10.1002/1873-3468.12040
M3 - Article
C2 - 26762170
AN - SCOPUS:84955557590
SN - 0014-5793
VL - 590
SP - 53
EP - 67
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -